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Applications of Flow Cytometry in Stem Cell Research and Tissue RegenerationFrom Wiley-Blackwell
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A much-needed primer on the use of laser flow cytometry for stem cell analysis
Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells.
Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction.
Chapter coverage includes:
- Stem cell analysis and sorting using side population
- Flow cytometry in the study of proliferation and apoptosis
- Stem cell biology and application
- Identification and isolation of very small embryonic-like stem cells from murine and human specimens
- Hematopoietic stem cells—issues in enumeration
- Human embryonic stem cells: long-term culture and cardiovascular differentiation
- Limbal stem cells and corneal regeneration
- Flow cytometric sorting of spermatogonial stem cells
- Breast cancer stem cells
- Stem cell marker expression in cells from body cavity fluids
This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
- Sales Rank: #3416027 in eBooks
- Published on: 2011-05-12
- Released on: 2011-05-12
- Format: Kindle eBook
Review
"Contributors from a wide range of medical and biological sciences explain methods and procedures of flow cytometry specific to its use in research on stem cells and related matters." (SciTech Book News, December 2010)
From the Back Cover
A much-needed primer on the use of laser flow cytometry for stem cell analysis
Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells.
Now, Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes:
-
Stem cell analysis and sorting using side population
-
Flow cytometry in the study of proliferation and apoptosis
-
Stem cell biology and application
-
Identification and isolation of very small embryonic-like stem cells from murine and human specimens
-
Hematopoietic stem cells—issues in enumeration
-
Human embryonic stem cells: long-term culture and cardiovascular differentiation
-
Limbal stem cells and corneal regeneration
-
Flow cytometric sorting of spermatogonial stem cells
-
Breast cancer stem cells
-
Stem cell marker expression in cells from body cavity fluids
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration is designed for graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. Laboratories engaged in stem cell research and use of stem cells for tissue regeneration will find this to be an invaluable source of information, as will any organization dealing in stem cell and tissue regeneration research.
About the Author
Dr. Satish Totey is among a select number of researchers who pioneered the isolation and characterization of pluripotent stem cells from human embryo.
Dr. Kaushik D. Deb is group leader and senior principal scientist, Human Embryonic Stem Cells Program, Stempeutics Research Pvt. Ltd. and Manipal Institute of Regenerative Medicine.
Most helpful customer reviews
0 of 0 people found the following review helpful.
Excellent resource for graduate students
By Nikonlensman
This is a nice & comprehensive protocol book for characterization of stem cells using flow cytometry. The focus is on methods to characterize cells & instrument set up with a brief background. Highly recommended
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